Resolution of Bacterial Proteins by Polyacrylamide Gel Electrophoresis on Slabs
نویسندگان
چکیده
منابع مشابه
Resolution of Bacterial Proteins by Polyacrylamide Gel Electrophoresis on Slabs
The application of a method of electrophoretic protein analysis to the investigation of the total protein composition of Salmonella typhimurium is described. It involves electrophoresis of many samples simultaneously on a thin slab of acrylamide gel, in the presence of sodium dodecyl sulfate, with the discontinuous buffer system of Laemmli ((19’70) Nature 227, 680485). Up to about 25 samples ca...
متن کاملResolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Membrane, soluble, and periplasmic fractions.
The application of a method of electrophoretic protein analysis to the investigation of the total protein composition of Salmonella typhimurium is described. It involves electrophoresis of many samples simultaneously on a thin slab of acrylamide gel, in the presence of sodium dodecyl sulfate, with the discontinuous buffer system of Laemmli ((19’70) Nature 227, 680485). Up to about 25 samples ca...
متن کاملPolyacrylamide gel electrophoresis of spinal-fluid proteins.
Twenty-three control samples of CSF were analyzed by vertical column electrophoresis on acrylamide gel. Quantitation results using a microdensitometer are presented. The normal protein pattern shows 15 constant bands which coincide in mobility and in many cases in staining pattern with serum proteins. The haptoglobin peaks, except for the band of the 1-1 type and the leading band of the 2-1 typ...
متن کاملSDS Polyacrylamide Gel Electrophoresis of Proteins.
Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes. The amount of SDS bound by a protein, and so the charge on the complex, is roughly proportional to its size. Common...
متن کاملMammalian melanosomal proteins: characterization by polyacrylamide gel electrophoresis.
For many years the major problem impeding the elucidation of the structure of melanin and the melanosome has been the insolubility of the melanin polymer. To date, alkaline and acid hydrolysis seem to be the only methods available which can completely solubilize the melanosome (1); unfortunately, both methods degrade the constituents of the organelle other than the melanin polymer. The recent e...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)43074-3